Utilization of Cassava Flour and Dorsata Honey as Alternative Medium for in Vitro Propagation of Banana

Shella B. Cacatian

Abstract


This study examined the possibilities of using Golden Yellow cassava flour and Dorsata honey for successful, inexpensive, and safe propagation protocol of banana of Lakatan variety in vitro. A one-factor experiment in a Completely Randomized Design was used to compare the growth of banana using different amounts of cassava flour and Dorsata honey. Multiple comparisons among the treatment mean revealed that in the four generations of the culture, the MS medium produced the tallest and the most vigorous shoots. It had the clearest appearance; however, it generated the least number of shoots and the highest percentage of contamination. Nonetheless, the treatment with 50 percent cassava flour possessed the best quality regarding gel strength. The alternative medium markedly reduced material costs to medium preparation. The combination of not greater than 50 percent of cassava flour and agar in the medium offers a suitable supporting surface for banana micropropagation, and it helps promote plant tissue culture (TC) techniques.


Keywords


alternative medium, banana, cassava flour, Dorsata honey, in vitro

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