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Email this article Characterization, Antimicrobial and Anti-fungal Activity of Endophytic Fungi Associated with Gelidiella acerosa (Forsskal) Feldmann and G. Hamel (1934) from the Intertidal Area of Silago, Southern Leyte, Philippines
Abstract
Endophytic fungi that live inside plant tissues are known to protect their hosts against pathogens. Thus this study was conducted to isolate, characterize and identify endophytic fungi from the red algae Gelidiella acerosa in the intertidal area of Balagawan, Silago, Southern Leyte and evaluate their antibacterial and antifungal activities. Endophytic fungi were isolated and cultured in Potato dextrose agar (PDA), characterized, identified up to division level only and were subjected to antibacterial and antifungal assays respectively. Eighteen endophytic fungi were isolated from Gelidiella acerosa from three sampling sites during a two sampling period. Results showed sixteen fungal isolates belonging to division Deuteromycota and one species each to divisions Ascomycota and Zygomycota. Escherichia coli and Bacillus subtilis were used in the antimicrobial assay as test organisms against the fungal isolates and Penicillin as the positive control. For the antifungal assay, Helminthosporium maydis and Sclerotium rolfsii (fungal pathogens) were used as the test organisms and Trichoderma harzianum as the positive control. Antibacterial assay results showed no significant difference between isolates (FI1 to FI18) for both E. coli and B. subtilis. Antifungal assay between isolates also showed no significant difference against the two pathogens tested. Moreover, identification of isolate to species level and verification of antibacterial and antifungal activity is recommended.
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Au, D.W.T., Jones, E.B.G. and Vrijmoed, L.L.P. (1999). Observations on the biology and ultrastructure of the asci and ascospores of Julella avicenniae from Malaysia. Mycological Research, 103:865-872.
Arnold, A. E., Z. Maynard, G. S., Gilbert, P. D., Coley, and T. A., Kursar. (2000). Are tropical fungal endophytes hyperdiverse? Ecology Letters 3:267-274.
Bell, D.K., Well, H.D., Markham, C.R. (1982). In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology, 73:9-382.
Bitacura, J. (2010). Biodegradation of Used Engine Oil Isolated from the Coastal Sediments in the Vicinity of Ormoc City Port Area. Publish BS Thesis. Visayas State University.
Booth, T. and Kenkel, N. (1986). Ecological studies of lignicolous marine fungi: a distribution model based on ordination and classification. In: The Biology of Marine Fungi (ed. S.T. Moss). Cambridge University Press, Cambridge: 297-310.
Carroll, G. (1998). Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symboint. Ecology 69:2-9
Dreyfuss, M.M. & Chapela, I.H. (1994). Potential of fungi in the discovery of novel, low molecular weight pharmaceuticals. In: The Discovery of Natural Products with Therapeutic Potential (ed. V.P. Gullo). Butterworth-Heinemann, London, UK: 49-80.
Fatope, O.M.(1995). Phytocompounds: Their Bioassay and Diversity. Discovery and Innovation, 7(3):229-236.
Fenical, W. (1975). Halogenation in the Rhodophyta. A review. J.Phycol. 11:245-259.
Frohlich, J. and Hyde, K.D. (1999). Biodiversity of palm fungi in the tropics: are global fungal diversity estimates realistic? Biodiversity and Conservation 8:977- 1004.
Gunatilaka, A.A.L., (2006). Natural products from plant-associated microorganisms: Distribution, structural diversity, bioactivity and implication of their occurrence. J. Nat. Prod, 69:509-526.
Govindarajulu, R, C. E. Hughes, and C. D. Bailey. (2011). Phylogenetic and population genetic assessment of diploid Leucaena reveal cryptic species diversity and patterns of allopatric divergent speciation. American Journal of Botany 98:2049-2063.
Google Earth. (2012). http://www.google.com/earth/download/ge/agree.html. Accessed on March 2013.
Hirsch, G.U. & Braun, U. (1992). Communities of parasitic microfungi. In: Handbook of Vegetation Science: Fungi in vegetation science, Vol. 19. (ed. W. Winterhoff). Kluwer Academic, Dordrecht, Netherlands: 225-250.
Hughes, G.C. (1974). Geographical distribution of the higher marine fungi. Veroeffentlichender Institute ftir Meeresforschung, Bremerhaven Supplement 5:419-441.
Hyde, K.D. and Lee, S.Y. (1995). Ecology of mangrove fungi and their role in nutrient cycling: 27 what gaps occur in our knowledge? Hydrobiologia 295:107-118.
Jones, E.B.G., Stanley, S.J., and Pinruan, U. (2008). Marine endophyte sources of new chemical natural products: a review. Bot. Mar. 51(3); 163-170.
Jones, E.B.G. and Alias, S.A. (1997). Biodiversity of mangrove fungi. In: Biodiversity of Tropical Microfungi (ed. K.D. Hyde). Hong Kong University Press, Hong Kong: 71-92.
Kjer, J., Debbab, A., Aly, A.H., and Proksch, P. (2010). Methods for isolation of marine derived endophytic fungi and their bioactive secondary products. Nat. Protoc. 5(3): 479-490.
Kohlmeyer, J., and Khlmeyer, E. (1979). Marine mycology: the higher fungi. Academic Press, New York, N.Y.
Kohlmeyer, J. (1983). Geography of marine fungi. Australian Journal of Botany Supplement Series 10: 67-76.
Li,W.C., Zhou, J., Gou L.D. (2007). Endophytic fungi associated with lichens in Baihua mountain of Beijing, China. Fungal Diversity 25:69-80.
Mao, S, Lee, S.J., Hwangbo, H., Kim, Y.W., Park, K.H., Cha, G.S., Park R.D., Kim, K.Y. (2006). Isolation and characterization of antifungal substance from Burk holderias. Culture broth. Curr. Microbiol. 53:358-364.
Newman, D.J., Cragg, G.M., Snader, K.M. (2003). Natural products as sources of new drugs over the period 1981-2002. A detailed analysis and description of current natural product derived therapeutic agents. J. Nat. Prod. 66:10-22
Regan, M.A., Glombitza, K.W. (1986). Handbook of Physiological Methods. Cambridge University Press; Cambridge, UK: 129-241.
Rao, P.P.S., (1995). Biological investigations of Indian phaeophyceae antimicrobial activity of frozen samples of genus Sargassum collected from Okha, West Coast of India. Seaweed Res. Utilisat., 17:105-109.
Shiomi, H.F., H.S.A. Silva, I.S. De Melo, F.V. Nunes and W. Bettiol. (2006). Bioprospecting endophytic bacteria for biological control of coffee leaf rust. Sci. Agric., 63(1):32-39.
Strobel, G.A., Manker, D.C., and Mercier, J. (2005). Endophytic fungi and methods ofuse. US Patent 6,911,338.9p.
The Municipality of Silago, Southern Leyte, Philippines Case Study: A Global Bright Spot forLand Use Planning2012. http://www.rareplanet.org/sites/rareplanet.org/_les/_nal_nalsilagocasestudy.pdf. Accessed on March 6, 2013.
Suryaranayanan, T.S.,Thirunavukkarasu N., Govindarajulu M.B., Sasse F., Jansen R., MuraliI T.S. 2009. Fungal endophytes and Bioprospecting. Fungal Biology Reviews (England) 23:9-19.
Suryaranayanan, T.S., Venkatavhalam, A.,Thirunavukkarasu, N., Ravishankar, J.P., Doble, M., Geetha, V. 2010 Internal mycobiota of marine macroalgae from the Tamilnadu coast: distribution, diversity and biotechnological potential. Botanica Marina 53:456-468.
Zuccaro, A., Schoch, C.L., Spatafora, J.W., Kohlmeyer, J., Draeger, S., and Mitchel, J.I. 2008. Detection and identification of fungi intimately associated with the brown seaweed Fucus serratus. Appl. Eviron. Microbiol. 74(4):931-941
DOI: 10.5281/jst.v5i1.55
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