Viability testing and characterization of the contaminants in probiotic animal feed supplement

Marianne Agnes T Mendoza, Julius V Abela


The popularity of probiotics has largely risen due to its numerous health effects. With the growth of probiotics as health supplement, applications to animal production systems have also gained momentum. This study aimed at assessing the contaminants present in a sample of probiotic animal feed supplement as well as evaluate the viability of the sample.

The concentration of associated microbes from the probiotic animal feed supplement was determined through dilution plating using potato dextrose agar (PDA) for molds and nutrient agar (NA) for bacteria. Colony counting was made after 3-4 days incubation for bacteria and 5-7 days for molds and yeasts. The cultural characteristics of the microbial colonies were described. Microscopic examination was also conducted to study the morphology of the microorganisms. Gram staining was done for the isolates. In addition, pH of the sample product was also determined.

Only yeast and 2 kinds of bacterial colony were found associated from the probiotic animal feed supplement. No mold growth was observed and the concentrations of these microorganisms. Colony growth of the isolates and shape of their cells were varied. The bacteria showed bluish violet color after Gram staining which indicates that they are Gram positive. The pH of the sample product was 3.4 or acidic. Studies assessing the effects of the probiotic sample to particular animal production systems should be undertaken to enrich the data gathered.


probiotic; animal feed supplement; microbiology


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