Application of Plackett-Burman Screening Design to Enhance Exopolysaccharide Production from Rhodotorula Yeast Strains

Mary Ann Jilly R. Ramirez, Ludito V. Ramirez


The genus Rhodotorula has the ability to produce exopolysaccharide (EPS), a promising compound known to have multifarious application in the industry. Plackett-Burman design (PBD) was used to efficiently screen important medium composition, fermentation conditions and highest producing Rhodotorula strains that would significantly increase EPS production. Out of the seven variables, the most effective factors showing high level of significance are as follows: the type of yeast preferring R. minuta BIOTECH 2178, high level of yeast extract and xylose, low level of glucose and shorter fermentation time. Rhodotorula minuta BIOTECH 2178 was found to be a prolific EPS producer with a maximum production of 2.130.48 g/L. Thus, the experimental design used in this study provide a rapid identification of important variables affecting EPS production. Subsequent optimization procedure of the significant parameters can be further explored using response surface methodology.


Fractional factorial design; Rhodotorula minuta BIOTECH 2178; response surface methodology


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