Application of Plackett-Burman Screening Design to Enhance Exopolysaccharide Production from Rhodotorula Yeast Strains

Mary Ann Jilly R. Ramirez, Ludito V. Ramirez

Abstract


The genus Rhodotorula has the ability to produce exopolysaccharide (EPS), a promising compound known to have multifarious application in the industry. Plackett-Burman design (PBD) was used to efficiently screen important medium composition, fermentation conditions and highest producing Rhodotorula strains that would significantly increase EPS production. Out of the seven variables, the most effective factors showing high level of significance are as follows: the type of yeast preferring R. minuta BIOTECH 2178, high level of yeast extract and xylose, low level of glucose and shorter fermentation time. Rhodotorula minuta BIOTECH 2178 was found to be a prolific EPS producer with a maximum production of 2.130.48 g/L. Thus, the experimental design used in this study provide a rapid identification of important variables affecting EPS production. Subsequent optimization procedure of the significant parameters can be further explored using response surface methodology.


Keywords


Fractional factorial design; Rhodotorula minuta BIOTECH 2178; response surface methodology

References


Bezeeraa MA, Santellia R, Oliveira EP, Villara LS, & Luciane AE (2008). Response surface methodology (RSM) as a tool for optimization in analytical. Talanta 76(5):965-970

Cho DH, Chae HJ & Kim EY (2001). Synthesis and characterization of a novel extracellular polysaccharide by Rhodotorula glutinis. Applied Biochemistry and Biotechnology 95:183-193

Ghada S, Manal I, Mahmoud G, Asker M, Ghay, EA. (2012). Production and biological evaluation of exopolysaccharide from isolated Rhodotorula glutinins. Australian Journal of Basic and Applied Sciences 6(3):401-408

Granato D, Calado VM (2013). The use and importance of design of experiments (DOE) in process modeling in food science and technology. In: Granato D. and Ares G. 2013. Mathematical and Statistical Methods in Food Science and Technology. Wiley-Blackwell .USA. 536 pages

Haroun B, El-Menoufy HA, Amin Ha, El-Waseif AA. (2013). Biosynthesis and Morphology of an Exopolysaccharide from a Probiotic Lactobacillus plantarum under different growth conditions.

Journal of Applied Sciences Research, 9(2):1256-1265

Lawson J. (2010). Design and Analysis of Experiment with SAS. Chapman and Hall/CRC Taylor and Francis Group. Florida, USA. 579 pages

Lee JH, Kim JH, Zhu IH (2001). Optimization of conditions for the production of pullulan and high molecular weight pullulan by Aureobasidium pullulans. Biotechnology Letters. 23(10):817-820

Madiedo P, Gavila NCG. (2005). Invited Review: Methods for the Screening Isolation and Characterization of Exopolysaccharides Produced by Lactic Acid Bacteria. Journal Dairy Science 88:843-856

Madiedo P., Gavila NCG. (2013). Fungal exopolysaccharide: production composition and applications. Microbiology Insights 2013:6 1-16.

Martinez C, Gertosio C, Labbe A, Perez R, Ganga MA, (2006). Production of Rhodotorula glutinis: a yeast that secretes a-L-arabinofuranosidase. Electronic Journal of Biotechnology 9:4

Pavlova K. Koleva M, Kratchanova M, Panchev I. (2005). Production and characterization of an exopolysaccharide yeast. World Journal of Microbiology and Biotechnology 20:435-439

Pawar ST, Bhosale AA, Gawade TB, Nale TR (2013). Isolation, screening and optimization of exopolysaccharide producing bacterium from saline soil. Journal of Microbiology and Biotechnology Research 3(3):24-31.

Peterson GR, Nelson GA, Cathey CA, Fuller G (1998). Rheologically interesting polysaccharides from yeasts. Applied Biochemistry and Biotechnology 20-21:845-67

Simova ED, Frengova GI, Beshkova DM (2004). Exopolysaccharides produced by mixed culture of yeast Rhodotorula rubra GED10 and yogurt bacteria (Streptococcus thermophilus 13a + Lactobacillus bulgaricus 2-11). Journal of Applied Microbiology 97:512-519

Sutherland IW (2001). Biofilm exopolysaccharide: a strong and sticky framework. Microbiology 147:3-9.

Velankar HR, Heble MR (2003). Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta. Electronic Journal of Biotechnology 2:90-103.

Yehia HM, Olayan EM, Elkhadragy MF, Khalaf-Allah AM, El-Shimi, NM (2013). Improvement of Carotenoid Pigments Produced by Rhodotorula glutinis. Life Science Journal 10(4):386-400.


Full Text: JST_2015 02

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.