Characterization, Antimicrobial and Anti-fungal Activity of Endophytic Fungi Associated with Gelidiella acerosa (Forsskal) Feldmann and G. Hamel (1934) from the Intertidal Area of Silago, Southern Leyte, Philippines

Berna Lou L. Aba, Neil Michael M. Almosa

Abstract


Endophytic fungi that live inside plant tissues are known to protect their hosts against pathogens. Thus this study was conducted to isolate, characterize and identify endophytic fungi from the red algae Gelidiella acerosa in the intertidal area of Balagawan, Silago, Southern Leyte and evaluate their antibacterial and antifungal activities. Endophytic fungi were isolated and cultured in Potato dextrose agar (PDA), characterized, identified up to division level only and were subjected to antibacterial and antifungal assays respectively. Eighteen endophytic fungi were isolated from Gelidiella acerosa from three sampling sites during a two sampling period. Results showed sixteen fungal isolates belonging to division Deuteromycota and one species each to divisions Ascomycota and Zygomycota. Escherichia coli and Bacillus subtilis were used in the antimicrobial assay as test organisms against the fungal isolates and Penicillin as the positive control. For the antifungal assay, Helminthosporium maydis and Sclerotium rolfsii (fungal pathogens) were used as the test organisms and Trichoderma harzianum as the positive control. Antibacterial assay results showed no significant difference between isolates (FI1 to FI18) for both E. coli and B. subtilis. Antifungal assay between isolates also showed no significant difference against the two pathogens tested. Moreover, identification of isolate to species level and verification of antibacterial and antifungal activity is recommended.


Keywords


endophytic fungi; antibacterial assay; antifungal assay; Gelidiella acerosa

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