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Cross-species Amplification and Characterization of Shorea Microsatellites in Shorea contorta Vidal (Dipterocarpaceae)

Randy Villarin, Kathleen Prinz, Teofanes Patindol, Reiner Finkeldey

Abstract


The tropical rainforests in Southeast Asia consist of diverse Dipterocarpaceae species. In the Philippines, Shorea contorta is one of the most economically and ecologically important dipterocarps. Presently, its long-term survival is critically endangered and the remaining genetic resources are assumed to be under pressure. In this study, we cross-amplify 23 Shorea microsatellites in S. contorta. The polymorphic loci were selected and characterized using planted and natural populations of the studied species. Results revealed a high success rate for cross-species amplication (78.26%). The ten (10) polymorphic microsatellites showed a mean number (Na) and eective number (Ne) of alleles of 6.00 and 2.75, respectively. The mean values for observed (Ho) and expected (He) heterozygosities were 0.45 and 0.47, respectively. In general, these microsatellites are useful for population genetic studies in S. contorta.

Keywords


Transferability; Cross-amplification; Dipterocarps; Simple Sequence Repeats; Tropical Forest;

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